EsturgeonV1, Main, Exploration, bibRecord, 001990

Rapid verification of meiotic gynogenesis and polyploidy in white sturgeon (Acipenser transmontanus Richardson)

Identifieur interne : 001990 ( Main/Exploration ); précédent : 001989; suivant : 001991

Rapid verification of meiotic gynogenesis and polyploidy in white sturgeon (Acipenser transmontanus Richardson)

Auteurs : A. L. Van Eenennaam [États-Unis] ; J. P. Van Eenennaam [États-Unis] ; J. F. Medrano [États-Unis] ; S. I. Doroshov [États-Unis]

Source :

Aquaculture [ 0044-8486 ] ; 1996.

RBID : Pascal:97-0142222

Descripteurs français

Pascal (Inist)
- Technique, Vérification, Gynogenèse, Polyploïdie, Marqueur RAPD, Marqueur génétique, Acipenser transmontanus, Acipenseridae.

English descriptors

KwdEn :
- Acipenser transmontanus, Genetic marker, Gynogenesis, Polyploidy, Random amplified polymorphic DNA, Technique, Verification.

Abstract

Copyright (c) 1996 Elsevier Science B.V. All rights reserved. We describe a novel random amplified polymorphic DNA (RAPD) based technique to rapidly assess the overall success of treatments designed to induce gynogenesis. To test this technique we produced white sturgeon (Acipenser transmontanus) meiotic gynogens. A total of 108 putative gynogens of known parentage from four different experimental treatments were screened using RAPD primers which were known to generate sire-specific markers. Only two individuals showed amplification of sire-specific markers indicating that they had received some paternal inheritance and were not true gynogens. This simple RAPD-based technique could be generally applicable for the verification of gynogenesis or androgenesis in other species, especially those which lack suitable phenotypic markers to trace the transmission of parental inheritance. We also determined the ploidy of 2469 diploid, triploid, tetraploid and mosaic sturgeon by using a Coulter Counter to analyze erythrocyte nuclei size and verified the results with flow cytometry. The Coulter Counter proved to be a rapid and accurate technique for ploidy analysis in sturgeon.

Affiliations:

Links toward previous steps (curation, corpus...)

to stream PascalFrancis, to step Corpus: 000479
to stream PascalFrancis, to step Curation: 000632
to stream PascalFrancis, to step Checkpoint: 000482
to stream Main, to step Merge: 001A99
to stream Main, to step Curation: 001990

Le document en format XML

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<front><div type="abstract" xml:lang="en">Copyright (c) 1996 Elsevier Science B.V. All rights reserved. We describe a novel random amplified polymorphic DNA (RAPD) based technique to rapidly assess the overall success of treatments designed to induce gynogenesis. To test this technique we produced white sturgeon (Acipenser transmontanus) meiotic gynogens. A total of 108 putative gynogens of known parentage from four different experimental treatments were screened using RAPD primers which were known to generate sire-specific markers. Only two individuals showed amplification of sire-specific markers indicating that they had received some paternal inheritance and were not true gynogens. This simple RAPD-based technique could be generally applicable for the verification of gynogenesis or androgenesis in other species, especially those which lack suitable phenotypic markers to trace the transmission of parental inheritance. We also determined the ploidy of 2469 diploid, triploid, tetraploid and mosaic sturgeon by using a Coulter Counter to analyze erythrocyte nuclei size and verified the results with flow cytometry. The Coulter Counter proved to be a rapid and accurate technique for ploidy analysis in sturgeon.</div>
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Rapid verification of meiotic gynogenesis and polyploidy in white sturgeon (Acipenser transmontanus Richardson)

Rapid verification of meiotic gynogenesis and polyploidy in white sturgeon (Acipenser transmontanus Richardson)

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri